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BRAF V600

Source

CIViC variant id 17: BRAF V600

Recipes that this example satisfies

None

Properties

id: civic.vid:17

CIViC Variant ID, where vid stands for Variant ID, derived from the Variant ID contained within the CIViC URL for this genomic alteration.

type: CategoricalVariant

This value is required by the specification for all Categorical Variant objects.

name: BRAF V600

Human-readable name for this variant.

description: BRAF V600 variants are missense amino acid substitutions that result in a change at valine 600, with V600E being the most common and V600K, V600M, V600R, and V600G also observed.

This field was populated with an example value because CIViC does not provide a longform description.

aliases: NM_004333.6(BRAF) V600, NM_004333.6 V600

Example aliases that include the MANE Select transcript for BRAF were included.

extensions: CIViC Representative Variant Coordinates

GRCh37 genomic coordinates on chromosome 7.

mappings: V600E (c.1799T>A), V600R (c.1798_1799delinsAG), V600K (c.1798_1799delinsAA), V600G (c.1799T>G), V600M (c.1798G>A)

ClinVar variants corresponding to amino acid substitutions at BRAF V600 were included as relatedMatch mappings.

Constraints

Defining Location Constraint

The defining location is amino acid position 600 within the BRAF protein (refseq:NP_004324.2), the protein product of the MANE Select coding transcript for BRAF (refseq:NM_004333.6), corresponding to valine in the reference sequence. The matchCharacteristic is set to “is_within”, meaning any variant whose affected residue falls within this position satisfies the constraint.

Members

The members field includes five VRS Allele objects generated using the VICC Variation Normalizer from the hgvs.c representations of BRAF:

  • V600E (NM_004333.6:c.1799T>A)

  • V600K (NM_004333.6:c.1798_1799delinsAA)

  • V600G (NM_004333.6:c.1799T>G)

  • V600M (NM_004333.6:c.1798G>A)

  • V600R (NM_004333.6:c.1798_1799delinsAG)

Full example: JSON

{
  "id": "civic.vid:17",
  "type": "CategoricalVariant",
  "name": "BRAF V600",
  "description": "BRAF V600 variants are missense amino acid substitutions that result in Valine changed. V600E is the most common substitution, but V600K, V600M, V600R, and V600G have also been observed.",
  "aliases": [
    "NM_004333.6(BRAF) V600",
    "NM_004333.6 V600"
  ],
  "extensions": [
    {
      "name": "CIViC Representative Variant Coordinates",
      "value": {
        "chromosome": "7",
        "start": 140453136,
        "stop": 140453137,
        "reference_bases": "",
        "variant_bases": "",
        "representative_transcript": "ENST00000288602.6",
        "ensembl_version": 75,
        "reference_build": "GRCh37"
      }
    }
  ],
  "constraints": [
    {
      "type": "DefiningLocationConstraint",
      "location": {
        "id": "ga4gh:SL.1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw",
        "description": "Amino acid position 600 within the BRAF protein (refseq:NP_004324.2), which is the protein product of the MANE Select (GRCh38) coding transcript for BRAF (refseq:NM_004333.6), corresponding to valine in the reference sequence.",
        "type": "SequenceLocation",
        "digest": "1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw",
        "sequenceReference": {
          "id": "refseq:NP_004324.2",
          "name": "NP_004324.2",
          "description": "The protein product of the MANE Select (GRCh38) coding transcript for BRAF (refseq:NM_004333.6), corresponding to the Refseq protein accession NP_004324.2.",
          "aliases": [
            "ensembl:ENSP00000288602.6",
            "ga4gh:SQ.cQvw4UsHHRRlogxbWCB8W-mKD4AraM9y"
          ],
          "type": "SequenceReference",
          "refgetAccession": "SQ.cQvw4UsHHRRlogxbWCB8W-mKD4AraM9y",
          "residueAlphabet": "aa"
        },
        "start": 599,
        "end": 600,
        "sequence": "V"
      },
      "relations": [
        {
          "primaryCoding": {
            "code": "translation_of",
            "system": "http://www.sequenceontology.org",
            "iris": [
              "http://www.sequenceontology.org/browser/current_release/term/translation_of"
            ]
          }
        }
      ],
      "matchCharacteristic": {
        "primaryCoding": {
          "code": "is_within",
          "system": "ga4gh-gks-term:location-match"
        }
      }
    }
  ],
  "mappings": [
    {
      "coding": {
        "system": "https://www.ncbi.nlm.nih.gov/clinvar/variation/",
        "code": "13961",
        "name": "NM_004333.6(BRAF):c.1799T>A (p.Val600Glu)",
        "iris": [
          "https://www.ncbi.nlm.nih.gov/clinvar/variation/13961"
        ]
      },
      "relation": "relatedMatch"
    },
    {
      "coding": {
        "system": "https://www.ncbi.nlm.nih.gov/clinvar/variation/",
        "code": "375940",
        "name": "NM_004333.6(BRAF):c.1798_1799delinsAG (p.Val600Arg)",
        "iris": [
          "https://www.ncbi.nlm.nih.gov/clinvar/variation/375940"
        ]
      },
      "relation": "relatedMatch"
    },
    {
      "coding": {
        "system": "https://www.ncbi.nlm.nih.gov/clinvar/variation/",
        "code": "375941",
        "name": "NM_004333.6(BRAF):c.1798_1799delinsAA (p.Val600Lys)",
        "iris": [
          "https://www.ncbi.nlm.nih.gov/clinvar/variation/375941"
        ]
      },
      "relation": "relatedMatch"
    },
    {
      "coding": {
        "system": "https://www.ncbi.nlm.nih.gov/clinvar/variation/",
        "code": "40389",
        "name": "NM_004333.6(BRAF):c.1799T>G (p.Val600Gly)",
        "iris": [
          "https://www.ncbi.nlm.nih.gov/clinvar/variation/40389"
        ]
      },
      "relation": "relatedMatch"
    },
    {
      "coding": {
        "system": "https://www.ncbi.nlm.nih.gov/clinvar/variation/",
        "code": "44815",
        "name": "NM_004333.6(BRAF):c.1798G>A (p.Val600Met)",
        "iris": [
          "https://www.ncbi.nlm.nih.gov/clinvar/variation/44815"
        ]
      },
      "relation": "relatedMatch"
    }
  ],
  "members": [
    {
      "id": "ga4gh:VA.W6xsV-aFm9yT2Bic5cFAV2j0rll6KK5R",
      "type": "Allele",
      "name": "NM_004333.6:c.1799T>A",
      "description": "VRS variation of NM_004333.6:c.1799T>A, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).",
      "digest": "W6xsV-aFm9yT2Bic5cFAV2j0rll6KK5R",
      "expressions": [
        {
          "syntax": "hgvs.c",
          "value": "NM_004333.6:c.1799T>A"
        }
      ],
      "location": {
        "id": "ga4gh:SL.8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U",
        "description": "Coding DNA (cDNA) position 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic position 140753336 on chromosome 7 (refseq:NC_000007.14, GRCh38).",
        "extensions": [
          {
            "name": "GRCh38 1-based, genomic position",
            "value": "chr7:140753336",
            "description": "Genomic position 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38)."
          },
          {
            "name": "GRCh38 1-based, inclusive interval",
            "value": "chr7:140753336-140753336",
            "description": "Genomic positions 140,753,336 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation."
          },
          {
            "name": "GRCh38 0-based, half-open interval",
            "value": "chr7:140753335-140753337",
            "description": "Genomic positions 140,753,335 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation."
          }
        ],
        "type": "SequenceLocation",
        "digest": "8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U",
        "sequenceReference": {
          "id": "refseq:NM_004333.6",
          "name": "NM_004333.6",
          "description": "The MANE Select (GRCh38) coding transcript for BRAF.",
          "aliases": [
            "ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa"
          ],
          "type": "SequenceReference",
          "refgetAccession": "SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa",
          "residueAlphabet": "na"
        },
        "start": 2024,
        "end": 2025,
        "sequence": "T"
      },
      "state": {
        "type": "LiteralSequenceExpression",
        "sequence": "A"
      }
    },
    {
      "id": "ga4gh:VA.GIOeMJX8rJ-q9ZGqmFRIUOGnXQ5NARht",
      "type": "Allele",
      "name": "NM_004333.6:c.1798_1799delinsAA",
      "description": "VRS variation of NM_004333.6:c.1798_1799delinsAA, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).",
      "digest": "GIOeMJX8rJ-q9ZGqmFRIUOGnXQ5NARht",
      "expressions": [
        {
          "syntax": "hgvs.c",
          "value": "NM_004333.6:c.1798_1799delinsAA"
        }
      ],
      "location": {
        "id": "ga4gh:SL.SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh",
        "description": "Coding DNA (cDNA) positions 1798 to 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic positions 140753336 to 140753337 on chromosome 7 (refseq:NC_000007.14, GRCh38).",
        "extensions": [
          {
            "name": "GRCh38 1-based, inclusive interval",
            "value": "chr7:140753336-140753337",
            "description": "Genomic positions 140,753,336 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation."
          },
          {
            "name": "GRCh38 0-based, half-open interval",
            "value": "chr7:140753335-140753337",
            "description": "Genomic positions 140,753,335 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation."
          }
        ],
        "type": "SequenceLocation",
        "digest": "SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh",
        "sequenceReference": {
          "id": "refseq:NM_004333.6",
          "name": "NM_004333.6",
          "description": "The MANE Select (GRCh38) coding transcript for BRAF.",
          "aliases": [
            "ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa"
          ],
          "type": "SequenceReference",
          "refgetAccession": "SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa",
          "residueAlphabet": "na"
        },
        "start": 2023,
        "end": 2025,
        "sequence": "GT"
      },
      "state": {
        "type": "LiteralSequenceExpression",
        "sequence": "AA"
      }
    },
    {
      "id": "ga4gh:VA.PEphL_4Hx5JYuJPMzgu0pRQIUcz_J9p0",
      "type": "Allele",
      "name": "NM_004333.6:c.1799T>G",
      "description": "VRS variation of NM_004333.6:c.1799T>G, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).",
      "digest": "PEphL_4Hx5JYuJPMzgu0pRQIUcz_J9p0",
      "expressions": [
        {
          "syntax": "hgvs.c",
          "value": "NM_004333.6:c.1799T>G"
        }
      ],
      "location": {
        "id": "ga4gh:SL.8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U",
        "description": "Coding DNA (cDNA) positions 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic position 140753336 on chromosome 7 (refseq:NC_000007.14, GRCh38).",
        "extensions": [
          {
            "name": "GRCh38 1-based, genomic position",
            "value": "chr7:140753336",
            "description": "Genomic position 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38)."
          },
          {
            "name": "GRCh38 1-based, inclusive interval",
            "value": "chr7:140753336-140753336",
            "description": "Genomic positions 140,753,336 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation."
          },
          {
            "name": "GRCh38 0-based, half-open interval",
            "value": "chr7:140753335-140753336",
            "description": "Genomic positions 140,753,335 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation."
          }
        ],
        "type": "SequenceLocation",
        "digest": "8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U",
        "sequenceReference": {
          "id": "refseq:NM_004333.6",
          "name": "NM_004333.6",
          "description": "The MANE Select (GRCh38) coding transcript for BRAF.",
          "aliases": [
            "ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa"
          ],
          "type": "SequenceReference",
          "refgetAccession": "SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa",
          "residueAlphabet": "na"
        },
        "start": 2024,
        "end": 2025,
        "sequence": "T"
      },
      "state": {
        "type": "LiteralSequenceExpression",
        "sequence": "G"
      }
    },
    {
      "id": "ga4gh:VA.O3Wd-kD48Dl1ihRUuf4vVybLaXYKFXnk",
      "name": "NM_004333.6:c.1798G>A",
      "description": "VRS variation of NM_004333.6:c.1799T>A, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).",
      "digest": "O3Wd-kD48Dl1ihRUuf4vVybLaXYKFXnk",
      "expressions": [
        {
          "syntax": "hgvs.c",
          "value": "NM_004333.6:c.1798G>A"
        }
      ],
      "type": "Allele",
      "location": {
        "id": "ga4gh:SL.1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw",
        "description": "Coding DNA (cDNA) position 1798 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic position 140753337 on chromosome 7 (refseq:NC_000007.14, GRCh38).",
        "extensions": [
          {
            "name": "GRCh38 1-based, genomic position",
            "value": "chr7:140753337",
            "description": "Genomic position 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38)."
          },
          {
            "name": "GRCh38 1-based, inclusive interval",
            "value": "chr7:140753337-140753337",
            "description": "Genomic positions 140,753,337 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation."
          },
          {
            "name": "GRCh38 0-based, half-open interval",
            "value": "chr7:140753336-140753337",
            "description": "Genomic positions 140,753,336 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation."
          }
        ],
        "type": "SequenceLocation",
        "digest": "1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw",
        "sequenceReference": {
          "id": "refseq:NM_004333.6",
          "name": "NM_004333.6",
          "description": "The MANE Select (GRCh38) coding transcript for BRAF.",
          "aliases": [
            "ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa"
          ],
          "type": "SequenceReference",
          "refgetAccession": "SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa",
          "residueAlphabet": "na"
        },
        "start": 2024,
        "end": 2025,
        "sequence": "G"
      },
      "state": {
        "type": "LiteralSequenceExpression",
        "sequence": "A"
      }
    },
    {
      "id": "ga4gh:VA.t97D17VsbiAa2U-znA-Qfx6DVInBd-_-",
      "type": "Allele",
      "name": "NM_004333.6:c.1798_1799delinsAG",
      "description": "VRS variation of NM_004333.6:c.1799T>A, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).",
      "digest": "t97D17VsbiAa2U-znA-Qfx6DVInBd-_-",
      "expressions": [
        {
          "syntax": "hgvs.c",
          "value": "NM_004333.6:c.1798_1799delinsAG"
        }
      ],
      "location": {
        "id": "ga4gh:SL.SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh",
        "description": "Coding DNA (cDNA) positions 1798 to 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic positions 140753336 to 140753337 on chromosome 7 (refseq:NC_000007.14, GRCh38).",
        "extensions": [
          {
            "name": "GRCh38 1-based, inclusive interval",
            "value": "chr7:140753336-140753337",
            "description": "Genomic positions 140,753,336 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation."
          },
          {
            "name": "GRCh38 0-based, half-open interval",
            "value": "chr7:140753335-140753337",
            "description": "Genomic positions 140,753,335 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation."
          }
        ],
        "type": "SequenceLocation",
        "digest": "SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh",
        "sequenceReference": {
          "id": "refseq:NM_004333.6",
          "name": "NM_004333.6",
          "description": "The MANE Select (GRCh38) coding transcript for BRAF.",
          "aliases": [
            "ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa"
          ],
          "type": "SequenceReference",
          "refgetAccession": "SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa",
          "residueAlphabet": "na"
        },
        "start": 2023,
        "end": 2025,
        "sequence": "GT"
      },
      "state": {
        "type": "LiteralSequenceExpression",
        "sequence": "AG"
      }
    }
  ]
}

Full example: YAML

id: civic.vid:17
type: CategoricalVariant
name: BRAF V600
description: >-
  BRAF V600 variants are missense amino acid substitutions that result in Valine changed.
  V600E is the most common substitution, but V600K, V600M, V600R, and V600G have also been observed.
aliases:
  - NM_004333.6(BRAF) V600
  - NM_004333.6 V600
extensions:
  - name: CIViC Representative Variant Coordinates
    value:
      chromosome: '7'
      start: 140453136
      stop: 140453137
      reference_bases: ''
      variant_bases: ''
      representative_transcript:  ENST00000288602.6
      ensembl_version: 75
      reference_build: GRCh37
constraints:
  - type: DefiningLocationConstraint
    location:
      id: ga4gh:SL.1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw
      description: Amino acid position 600 within the BRAF protein (refseq:NP_004324.2), which is the protein product of the MANE Select (GRCh38) coding transcript for BRAF (refseq:NM_004333.6), corresponding to valine in the reference sequence.
      type: SequenceLocation
      digest: 1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw
      sequenceReference:
        id: refseq:NP_004324.2
        name: NP_004324.2
        description: The protein product of the MANE Select (GRCh38) coding transcript for BRAF (refseq:NM_004333.6), corresponding to the Refseq protein accession NP_004324.2.
        aliases:
          - ensembl:ENSP00000288602.6
          - ga4gh:SQ.cQvw4UsHHRRlogxbWCB8W-mKD4AraM9y
        type: SequenceReference
        refgetAccession: SQ.cQvw4UsHHRRlogxbWCB8W-mKD4AraM9y
        residueAlphabet: aa
      start: 599
      end: 600
      sequence: V
    relations:
      - primaryCoding:
          code: translation_of
          system: http://www.sequenceontology.org
          iris:
            - http://www.sequenceontology.org/browser/current_release/term/translation_of
    matchCharacteristic:
      primaryCoding:
        code: is_within
        system: ga4gh-gks-term:location-match
mappings:
  - coding:
      system: https://www.ncbi.nlm.nih.gov/clinvar/variation/
      code: '13961'
      name: NM_004333.6(BRAF):c.1799T>A (p.Val600Glu)
      iris:
        - https://www.ncbi.nlm.nih.gov/clinvar/variation/13961
    relation: relatedMatch
  - coding:
      system: https://www.ncbi.nlm.nih.gov/clinvar/variation/
      code: '375940'
      name: NM_004333.6(BRAF):c.1798_1799delinsAG (p.Val600Arg)
      iris:
        - https://www.ncbi.nlm.nih.gov/clinvar/variation/375940
    relation: relatedMatch
  - coding:
      system: https://www.ncbi.nlm.nih.gov/clinvar/variation/
      code: '375941'
      name: NM_004333.6(BRAF):c.1798_1799delinsAA (p.Val600Lys)
      iris:
        - https://www.ncbi.nlm.nih.gov/clinvar/variation/375941
    relation: relatedMatch
  - coding:
      system: https://www.ncbi.nlm.nih.gov/clinvar/variation/
      code: '40389'
      name: NM_004333.6(BRAF):c.1799T>G (p.Val600Gly)
      iris:
        - https://www.ncbi.nlm.nih.gov/clinvar/variation/40389
    relation: relatedMatch
  - coding:
      system: https://www.ncbi.nlm.nih.gov/clinvar/variation/
      code: '44815'
      name: NM_004333.6(BRAF):c.1798G>A (p.Val600Met)
      iris:
        - https://www.ncbi.nlm.nih.gov/clinvar/variation/44815
    relation: relatedMatch
members:
  - id: ga4gh:VA.W6xsV-aFm9yT2Bic5cFAV2j0rll6KK5R
    type: Allele
    name: NM_004333.6:c.1799T>A
    description: VRS variation of NM_004333.6:c.1799T>A, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).
    digest: W6xsV-aFm9yT2Bic5cFAV2j0rll6KK5R
    expressions:
      - syntax: hgvs.c
        value: NM_004333.6:c.1799T>A
    location:
      id: ga4gh:SL.8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U
      description: Coding DNA (cDNA) position 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic position 140753336 on chromosome 7 (refseq:NC_000007.14, GRCh38).
      extensions:
        - name: GRCh38 1-based, genomic position
          value: chr7:140753336
          description: Genomic position 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38).
        - name: GRCh38 1-based, inclusive interval
          value: chr7:140753336-140753336
          description: Genomic positions 140,753,336 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation.
        - name: GRCh38 0-based, half-open interval
          value: chr7:140753335-140753337
          description: Genomic positions 140,753,335 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation.
      type: SequenceLocation
      digest: 8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U
      sequenceReference:
        id: refseq:NM_004333.6
        name: NM_004333.6
        description: The MANE Select (GRCh38) coding transcript for BRAF.
        aliases:
          - ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        type: SequenceReference
        refgetAccession: SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        residueAlphabet: na
      start: 2024
      end: 2025
      sequence: T
    state:
      type: LiteralSequenceExpression
      sequence: A
  - id: ga4gh:VA.GIOeMJX8rJ-q9ZGqmFRIUOGnXQ5NARht
    type: Allele
    name: NM_004333.6:c.1798_1799delinsAA
    description: VRS variation of NM_004333.6:c.1798_1799delinsAA, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).
    digest: GIOeMJX8rJ-q9ZGqmFRIUOGnXQ5NARht
    expressions:
      - syntax: hgvs.c
        value: NM_004333.6:c.1798_1799delinsAA
    location:
      id: ga4gh:SL.SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh
      description: Coding DNA (cDNA) positions 1798 to 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic positions 140753336 to 140753337 on chromosome 7 (refseq:NC_000007.14, GRCh38).
      extensions:
        - name: GRCh38 1-based, inclusive interval
          value: chr7:140753336-140753337
          description: Genomic positions 140,753,336 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation.
        - name: GRCh38 0-based, half-open interval
          value: chr7:140753335-140753337
          description: Genomic positions 140,753,335 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation.
      type: SequenceLocation
      digest: SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh
      sequenceReference:
        id: refseq:NM_004333.6
        name: NM_004333.6
        description: The MANE Select (GRCh38) coding transcript for BRAF.
        aliases:
          - ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        type: SequenceReference
        refgetAccession: SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        residueAlphabet: na
      start: 2023
      end: 2025
      sequence: GT
    state:
      type: LiteralSequenceExpression
      sequence: AA
  - id: ga4gh:VA.PEphL_4Hx5JYuJPMzgu0pRQIUcz_J9p0
    type: Allele
    name: NM_004333.6:c.1799T>G
    description: VRS variation of NM_004333.6:c.1799T>G, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).
    digest: PEphL_4Hx5JYuJPMzgu0pRQIUcz_J9p0
    expressions:
      - syntax: hgvs.c
        value: NM_004333.6:c.1799T>G
    location:
      id: ga4gh:SL.8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U
      description: Coding DNA (cDNA) positions 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic position 140753336 on chromosome 7 (refseq:NC_000007.14, GRCh38).
      extensions:
        - name: GRCh38 1-based, genomic position
          value: chr7:140753336
          description: Genomic position 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38).
        - name: GRCh38 1-based, inclusive interval
          value: chr7:140753336-140753336
          description: Genomic positions 140,753,336 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation.
        - name: GRCh38 0-based, half-open interval
          value: chr7:140753335-140753336
          description: Genomic positions 140,753,335 to 140,753,336 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation.
      type: SequenceLocation
      digest: 8HBKs9fzlT3tKWlM03REjkg_0Om6Y33U
      sequenceReference:
        id: refseq:NM_004333.6
        name: NM_004333.6
        description: The MANE Select (GRCh38) coding transcript for BRAF.
        aliases:
          - ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        type: SequenceReference
        refgetAccession: SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        residueAlphabet: na
      start: 2024
      end: 2025
      sequence: T
    state:
      type: LiteralSequenceExpression
      sequence: G
  - id: ga4gh:VA.O3Wd-kD48Dl1ihRUuf4vVybLaXYKFXnk
    name: NM_004333.6:c.1798G>A
    description: VRS variation of NM_004333.6:c.1799T>A, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).
    digest: O3Wd-kD48Dl1ihRUuf4vVybLaXYKFXnk
    expressions:
      - syntax: hgvs.c
        value: NM_004333.6:c.1798G>A
    type: Allele
    location:
      id: ga4gh:SL.1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw
      description: Coding DNA (cDNA) position 1798 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic position 140753337 on chromosome 7 (refseq:NC_000007.14, GRCh38).
      extensions:
        - name: GRCh38 1-based, genomic position
          value: chr7:140753337
          description: Genomic position 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38).
        - name: GRCh38 1-based, inclusive interval
          value: chr7:140753337-140753337
          description: Genomic positions 140,753,337 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation.
        - name: GRCh38 0-based, half-open interval
          value: chr7:140753336-140753337
          description: Genomic positions 140,753,336 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation.
      type: SequenceLocation
      digest: 1ReS370wzmC9PGTn13XrnG8Ot7UAKYuw
      sequenceReference:
        id: refseq:NM_004333.6
        name: NM_004333.6
        description: The MANE Select (GRCh38) coding transcript for BRAF.
        aliases:
          - ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        type: SequenceReference
        refgetAccession: SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        residueAlphabet: na
      start: 2024
      end: 2025
      sequence: G
    state:
      type: LiteralSequenceExpression
      sequence: A
  - id: ga4gh:VA.t97D17VsbiAa2U-znA-Qfx6DVInBd-_-
    type: Allele
    name: NM_004333.6:c.1798_1799delinsAG
    description: VRS variation of NM_004333.6:c.1799T>A, generated with the VICC Variation Normalizer (https://github.com/cancervariants/variation-normalization).
    digest: t97D17VsbiAa2U-znA-Qfx6DVInBd-_-
    expressions:
      - syntax: hgvs.c
        value: NM_004333.6:c.1798_1799delinsAG
    location:
      id: ga4gh:SL.SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh
      description: Coding DNA (cDNA) positions 1798 to 1799 of the BRAF MANE Select coding transcript (refseq:NM_004333.6), corresponding to genomic positions 140753336 to 140753337 on chromosome 7 (refseq:NC_000007.14, GRCh38).
      extensions:
        - name: GRCh38 1-based, inclusive interval
          value: chr7:140753336-140753337
          description: Genomic positions 140,753,336 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 1-based, inclusive interval notation.
        - name: GRCh38 0-based, half-open interval
          value: chr7:140753335-140753337
          description: Genomic positions 140,753,335 to 140,753,337 on chromosome 7 (refseq:NC_000007.14, GRCh38); 0-based, half-open interval notation.
      type: SequenceLocation
      digest: SXY8ShFrBAfSvtO_sXLMQKN3Ozc5awgh
      sequenceReference:
        id: refseq:NM_004333.6
        name: NM_004333.6
        description: The MANE Select (GRCh38) coding transcript for BRAF.
        aliases:
          - ga4gh:SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        type: SequenceReference
        refgetAccession: SQ.aKMPEJgmlZXt_F6gRY5cUG3THH2n-GUa
        residueAlphabet: na
      start: 2023
      end: 2025
      sequence: GT
    state:
      type: LiteralSequenceExpression
      sequence: AG